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Mildly Alkaline Ionized Water: Characteristics, Benefits, and Future

Mildly Alkaline Ionized Water

Mildly alkaline water (pH 8-10) is marketed as healthy drinking water.1 It is produced from water ionizers via electrolysis.2 Alkaline water containing molecular hydrogen is produced at the negative electrode (cathode).3 There are many names given to this water including: alkaline water,4 ionized water,5 alkali ion water,6 cathodic water,7  electrolyzed reduced water,9 and many more.

Electrolyzed reduced water (ERW) is the most common term in the scientific literature.1 It is called “electrolyzed” because the water has undergone electrolysis and is called “reduced” because the water at the cathode has been reduced to hydrogen gas and hydroxides. This alkaline water has reductive characteristics due to the presence of dissolved hydrogen gas.

 

Alkaline Ionized Water Characteristics

ERW for drinking purposes has a pH between 8-10 and a negative oxidation-reduction potential between -100 mV to -700 mV.  It contains dissolved molecular hydrogen gas (responsible for the –ORP) and low dissolved molecular oxygen gas.3

 

Emerging Health Benefits

Studies on ERW began in the 1930’s in Japan1 and in 1965, the Japanese Ministry of Health, Labor, and Welfare approved ERW as a medical substance10 with potential to improve gastrointestinal symptoms11 (Read the details about this here). Over the ensuing decades, anecdotal and scientific evidence accumulated substantiating many other benefits of ERW1 such as; protecting DNA from radical damage,12 increasing glucose uptake,13 improving diabetes,14 preventing premature cell death,15 offering liver protection,16 preventing lipid oxidation,17 and others. 18

However, it was uncertain as to how ERW was producing these benefits.19-20 Some marketers,8 and unfortunately some scientists,1 started promoting ideas like microclustering, reduced surface tension, negatively charged water molecules, free electrons, active hydrogen,1 and others.

*See ERW Skeptics and Pseudoscience

 

Molecular Hydrogen is Key to ERW’s Health Effects

However, all these ideas have failed to pass scientific scrutiny,21-25 leaving the beneficial empirical and scientific observations without a rational explanation.21 It is now well-recognized that the primary agent responsible for the benefits is attributed to the dissolved molecular hydrogen gas.26 One leading ERW researcher from Kyushu University tested all the properties of ERW and the only one that exerted a therapeutic effect was the molecular hydrogen.27 This same observation was made by one of our advisors Dr. Lee from Korea who was a pioneer in ionized water research in the 1990s and now the leading Korean hydrogen scientist, as well as the the President of the Korean water society. He made the following statement:

About 17 years ago (1990’s) I began studying alkaline ionized water and published scientific articles on its antioxidant, anticancer, and anti-diabetic effects, but did not really understand why the water worked. It was difficult to believe. Upon further investigation I have now confirmed that the benefits from the alkaline ionized water are attributed to the hydrogen gas produced during electrolysis. The more I research and learn about hydrogen the more dedicated and passionate I become in educating and helping others.

The first time a link was suggested between ERW and hydrogen (although this was atomic or [“active”] hydrogen) appears to be in 1995.28 However, molecular hydrogen was not established and well-recognized as therapeutic until 2007.29 Prior to this time, articles on ERW made no mention of molecular hydrogen—at least not as an active component.30 However, starting in about 2010, articles on ERW began focusing almost exclusively on molecular hydrogen.30 Many recent articles will include molecular hydrogen in the title of the article; for example, “…molecular hydrogen saturated alkaline electrolyzed water…”.31

Evidence

This is also clearly illustrated in a recently published article.32 The researchers used fresh ERW at pH 8.5 and 9.5 with a low H2 concentration in one group, and ERW with a pH of 8.5 and 9.5 with additional H2 gas (via bubbling) in another group.  The results show that the ERW with a low H2 concentration did not offer any therapeutic effects.  However, the ERW at pH 8.5 and 9.5, with the additional H2 gas, provided significant therapeutic protection.

Importantly there was not a statistically significant difference between the pH 8.5 and 9.5 with the same higher H2 concentrations.  This further shows that it is the dissolved molecular hydrogen that is responsible for the therapeutic effects of ERW and not the alkaline pH or any other enigmatic properties.

In fact it is so well recognized that molecular hydrogen is the key component of ERW, that some studies will remove the molecular hydrogen from the ERW and use it for the control group.27, 40-42

For example, this research group40 prepared three types of waters: filtered water FW, electrolyzed reduced water ER, and ER with the H2 dehydrogenated (removed) DR. They made fresh samples twice daily and delivered it by a metallic straw from a closed bottle.

Screen Shot 2014-08-08 at 9.44.56 PM

The table to the left shows the properties of these types of waters. It was observed in the study that the only type of water that provided the protective effects was the ER containing the dissolved hydrogen gas. Just a note, the ORP was only -148 mV, which one would expect it to be much more negative at that pH and H2 concentration. However having done this for so long, I realize that ORP is an invalid measurement. I have tested ERW and received similar results and then tested it again after cleaning the probe and got -800 mV (see this article on ORP). So the lower ORP should not be a concerning issue. The water ionizer they used was a top commercial water ionizer in Japan TRIM ION TI-9000 system (Nihon Trim), which is also certified as a medical device.

Another study41 confirmed that it is the hydrogen gas and not the pH or minerals, or other properties of the alkaline reduced water that is responsible for the therapeutic benefits. They used three waters: regular water (RW) pH ?7, degassed alkaline reduced water (DW) pH ?9.5, and hydrogen alkaline reduced water (HW) pH ?9.5. Their results (see below) showed that only the water containing the hydrogen gas was effective at reducing various metalloproteinase enzymes (MMP2, MMP3), markers of oxidative stress (nitrotyrosine, 4-HNE, 8OHdG, MDA), vascular inflammation (ICAM, ETA, TNF?, IL-6), intimal hyperplasia and macrophage infiltration (ED1).

Screen Shot 2014-10-07 at 3.37.20 PM

 

One more study42 that helps illustrate that it is the hydrogen gas that is responsible for the therapeutic effects of ionized water, is one by Ignacio and colleagues. These researchers used four types of water:

Four groups of hairless mice were exposed to UVB radiation and then each group was bathed in one of these different waters.  The results show that only the ionized water with the higher hydrogen gas concentration increased the antioxidant activity of glutathione peroxidase (GPx), and reduced inflammatory cytokines (IL-12p70, IL-1?, IFN-?), as shown in the below figures.

 

Screen Shot 2014-10-08 at 3.41.26 PM

 

 

 

 

Consumer Experience

Consumers of ERW already know that the water is most effective when ingested as fresh as possible. This is because the molecular hydrogen starts to leave the water (as seen by the loss of the –ORP).  Researchers now administer the water as fresh as possible, as well as use other methods to increase the concentration of molecular hydrogen. 33Another method researchers are now choosing to produce molecular hydrogen-rich water is simply bubbling molecular hydrogen gas into pure water.37 This also eliminates any potential variables and assures saturation is achieved.

Not all machines are created equal.

Lastly, one recent review article43 specifically states that studies/researchers have “explicitly proved that molecular hydrogen but not alkaline in the electrolyzed alkaline water exerts therapeutic effects”. This information is important to the ionized water industry and consumer because it increases the importance of knowing and having water with a decent hydrogen gas concentration. Not all machines are created equal, and perhaps differences in H2 concentrations (not ORP or pH) is responsible for differences in the observed therapeutic effects.32

Molecular Hydrogen Concentration in ERW

Some types of electrolysis units are manufactured to produce water that is saturated (1.6 ppm)31 or even supersaturated (2-3 ppm)34 with molecular hydrogen by these are often not conventional alkaline water ionizers.  Some may be a batch type machine31 that maintains a neutral pH, but saturates the water with molecular hydrogen or a flow through system that can also maintain neutral pH.  Water ionizers produce alkaline water  and depending on a number of factors (e.g. flow rate, conductivity, etc) can produce between 0.05 to over 2 ppm.31,34-36 However, many machines only produce about a 6% saturated level (0.1 ppm) at a pH of 9.5.36  By running the water very slowly, these machines may increase the molecular hydrogen concentration, but the resulting pH is above 11.0 making the water unpalatable.36 

The ability to produce high concentrations of molecular hydrogen at a palatable pH (less than 9.5 or neutral), and what is required to maintain this concentration are an important parameters when considering one of these machines.35

The Future

Although the evidence31-32, 38-43 is overwhelming that molecular hydrogen is the key to ERW’s benefits, most water ionizer companies make no mention of molecular hydrogen as a selling point.  Not only do they not know what their water’s molecular hydrogen concentration is, but also they do not even know that it is the molecular hydrogen that is responsible for the benefits. This is simply because of the fact the importance of H2 was not even known by the scientists until 2007, which is decades after water ionizers were developed. Importantly, the market is generally about 10 years behind the science. Since molecular hydrogen was established in 2007, ten years from that time, about 2017, will be the year that the market will really start to take off,  exponentially promulgating the benefits of molecular hydrogen.

 

References: Click Here

  1. SHIRAHATA, S., HAMASAKI, T. & TERUYA, K. (2012). Advanced research on the health benefit of reduced water. Trends in Food Science & Technology 23, 124-131.
  2. KUMON, K. (1997). What Is Functional Water? Artificial Organs 21, 2-4.
  3. SHIRAHATA, S., KABAYAMA, S., NAKANO, M., MIURA, T., KUSUMOTO, K., GOTOH, M., HAYASHI, H., OTSUBO, K., MORISAWA, S. & KATAKURA, Y. (1997). Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage. Biochemical and Biophysical Research Communications 234, 269-274.
  4. MERNE, M. E., SYRJANEN, K. J. & SYRJANEN, S. M. (2001). Systemic and local effects of long-term exposure to alkaline drinking water in rats. Int J Exp Pathol 82, 213-9.
  5. YOKOYAMA, J.-M. K. A. K. (1997). Effects of alkaline ionized water on spontaneously diabetic GK-rats fed sucrose. Korea. J. of Lab. Anim Sa 13, 187-190.
  6. KIKUCHI, K., TAKEDA, H., RABOLT, B., OKAYA, T., OGUMI, Z., SAIHARA, Y. & NOGUCHI, H. (2001). Hydrogen particles and supersaturation in alkaline water from an Alkali-Ion-Water electrolyzer. Journal of Electroanalytical Chemistry 506, 22-27.
  7. BERJAK, P., SERSHEN, VARGHESE, B. & PAMMENTER, N. W. (2011). Cathodic amelioration of the adverse effects of oxidative stress accompanying procedures necessary for cryopreservation of embryonic axes of recalcitrant-seeded species. Seed Science Research 21, 187-203.
  8. Trademark Name of Enagic for ionized water http://www.enagic.com
  9. KOMATSU, T., KATAKURA, Y., TERUYA, K., OTSUBO, K., MORISAWA, S., & & SHIRAHATA, S. (2003). Electrolyzed reduced water induces differentiation in K-562 human leukemia cells. Animal cell technology: Basic & applied aspects, 387-391.
  10. Nishimoto right child "alkaline ionized water", "Comprehensive Dictionary of water", Maruzen, 2009, 19 pages, ISBN 978-4-621-08040-5 .
  11. (March 30, 1998) Issue # 318 Ministry of Health and Welfare Pharmaceutical and Medical Safety Director notification pharmaceutical departure (National Institute of Health Sciences)
  12. LEE, M. Y., KIM, Y. K., RYOO, K. K., LEE, Y. B. & PARK, E. J. (2006). Electrolyzed-reduced water protects against oxidative damage to DNA, RNA, and protein. Appl Biochem Biotechnol 135, 133-44.
  13. M. ODA, K. K., K. TERUYA, T. HARA, T. MAKI, S. KABAYAMA, Y. KATAKURA, K. OTSUBO, S. MORISAWA, H. HAYASHI. (2000). Electrolyzed and natural reduced water exhibit insulin-like activity on glucose uptake into muscle cells and adipocytes. Animal Cell Technology: Products from Cells, Cells as Products, 425-427.
  14. SHIRAHATA, S. (2001). Anti-oxidative water improves diabetes. Animal Cell Technology: from target to market. Proceedings of the 17th ESACT meeting, June 10-14, 2001 p. 574-577
  15. T. KASHIWAGI, T. H., S. KABAYAMA, M. TAKAKI, K. TERUYA, Y. KATAKURA, K. OTUBO, S. MORISAWA, S. SHIRAHATA. (2005). Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water. Animal Cell Technology Meets Genomics 2, 257-260.
  16. TSAI, C. F., HSU, Y. W., CHEN, W. K., CHANG, W. H., YEN, C. C., HO, Y. C. & LU, F. J. (2009). Hepatoprotective effect of electrolyzed reduced water against carbon tetrachloride-induced liver damage in mice. Food Chem Toxicol 47, 2031-6.
  17. MASUMI ABE, S. S., KAZUKO TOH, TAKEKI HAMASAKI, NOBORU NAKAMICHI, KIICHIRO TERUYA, YOSHINORI KATAKURA, SHINKATSU MORISAWA, SANETAKA SHIRAHATA. (2010). Suppressive Effect of ERW on Lipid Peroxidation and Plasma Triglyceride Level. Animal Cell Technology: Basic & Applied Aspects 16.
  18. S. SHIRAHATA, S. K., K. KUSUMOTO, M. GOTOH, K. TERUYA, K. OTSUBO, J. S. MORISAWA, H. HAYASHI, K. KATAKURA. (2002). Electrolyzed Reduced Water Which Can Scavenge Active Oxygen Species Supresses Cell Growth and Regulates Gene Expression of Animal Cells. New Developments and New Applications in Animal Cell Technology, 93-96.
  19. Association of Alkaline Ionized Water Apparatus. History
  20. Miyashita, Kazuo, et al. (2003) "Antioxidant Activity of Electrolized Sodium Chloride." Book Food Factors in Health Promotion and Disease Prevention. P. 274-288. American Chemical Society Symposium Series, V. 851
  21. HIRAOKA, A., TAKEMOTO, M., SUZUKI, T., SHINOHARA, A., CHIBA, M., SHIRAO, M. & YOSHIMURA, Y. (2004). Studies on the properties and real existence of aqueous solution systems that are assumed to have antioxidant activities by the action of "active hydrogen"'. Journal of Health Science 50, 456-465.
  22. RHEEM, K. E., LIM, Y. R., LEE, B. S., LEE, S. K., SEONG, K. M., HYUN, D. H. & MIN, K. J. (2012). Does alkaline-reduced hexagonal water delay the aging process in Drosophila? Geriatr Gerontol Int 12, 151-4.
  23. Hairston, James E., et al. "Drinking water and water treatment scams." Auburn, AL: Auburn University, Agronomy and Soils Department, Water Quality Timely Information, October 22 (2003).
  24. Shermer, Michael. "Bunkum!." Scientific American 290.1 (2004): 36-36.
  25. Binstock, Robert H. "Anti-aging medicine: The history anti-aging medicine and research: A realm of conflict and profound societal implications." The Journals of Gerontology Series A: Biological Sciences and Medical Sciences 59.6 (2004): B523-B533.
  26. YAN, H., KASHIWAKI, T., HAMASAKI, T., KINJO, T., TERUYA, K., KABAYAMA, S. & SHIRAHATA, S. (2011). The neuroprotective effects of electrolyzed reduced water and its model water containing molecular hydrogen and Pt nanoparticles. BMC Proc 5 Suppl 8, P69.
  27. Personal communication.
  28. Hayashi, H. (1995) Explore 6, 28–31.
  29. OHTA, S. (2008). [Hydrogen gas and hydrogen water act as a therapeutic and preventive antioxidant with a novel concept]. Nihon Ronen Igakkai Zasshi 45, 355-62.
  30. A reading of all pre 2007 articles and post 2007 articles clearly illustrates this fact.
  31. FUJITA, R., TANAKA, Y., SAIHARA, Y., YAMAKITA, M., ANDO, D. & KOYAMA, K. (2011). Effect of molecular hydrogen saturated alkaline electrolyzed water on disuse muscle atrophy in gastrocnemius muscle. Journal of Physiological Anthropology 30, 195-201.
  32. Xue, Jinling, Guodong Shang, Yoshinori Tanaka, Yasuhiro Saihara, Lingyan Hou, Natalia Velasquez, Wenjun Liu, and Yun Lu. "Dose-dependent inhibition of gastric injury by hydrogen in alkaline electrolyzed drinking water." BMC complementary and alternative medicine 14, no. 1 (2014): 81.
  33. LI, Y., HAMASAKI, T., NAKAMICHI, N., KASHIWAGI, T., KOMATSU, T., YE, J., TERUYA, K., ABE, M., YAN, H., KINJO, T., KABAYAMA, S., KAWAMURA, M. & SHIRAHATA, S. (2011). Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus. Cytotechnology 63, 119-31.
  34. KIKUCHI, K., TAKEDA, H., RABOLT, B., OKAYA, T., OGUMI, Z., SAIHARA, Y. & NOGUCHI, H. (2001). Hydrogen particles and supersaturation in alkaline water from an Alkali-Ion-Water electrolyzer. Journal of Electroanalytical Chemistry 506, 22-27.
  35. TANAKA, Y., UCHINASHI, S., SAIHARA, Y., KIKUCHI, K., OKAYA, T. & OGUMI, Z. (2003). Dissolution of hydrogen and the ratio of the dissolved hydrogen content to the produced hydrogen in electrolyzed water using SPE water electrolyzer. Electrochimica Acta 48, 4013-4019.
  36. Testing performed by AquaSciences LLC
  37. GUO, J. D., LI, L., SHI, Y. M., WANG, H. D. & HOU, S. X. (2013). Hydrogen water consumption prevents osteopenia in ovariectomized rats. Br J Pharmacol 168, 1412-20.
  38. OHNO, K., ITO, M. & ICHIHARA, M. (2012). Molecular hydrogen as an emerging therapeutic medical gas for neurodegenerative and other diseases. Oxidative Medicine and Cellular Longevity 2012, 353152.
  39. ZHANG, J. Y., LIU, C., ZHOU, L., QU, K., WANG, R. T., TAI, M. H., LEI, J. C. W. L., WU, Q. F. & WANG, Z. X. (2012). A Review of Hydrogen as a New Medical Therapy. Hepato-Gastroenterology 59, 1026-1032.
  40. Wan-Jun Zhu1, Masaaki Nakayama, Takefumi Mori1, Kiyotaka Hao, Hiroyuki Terawaki, Junichiro Katoh1, Shigeru Kabayama, and Sadayoshi Ito. (2013). Amelioration of cardio-renal injury with aging in dahl salt-sensitive rats by H2-enriched electrolyzed water. Medical Gas Research. 3:26
  41. Sun, Q., Kawamura, T., Masutani, T. Masutani, K., Peng, X. Stolz, D.B. Pribis, J.P. Billiar, T. R., Sun, X., Bemudez, C. A., Toyoda, Y., Nakao, A. (2012). Oral intake of hydrogen-rich water inhibits intimal hyperplasia in arterialized vein grafts in rats. Cardiovasc Res. 94, (1) 144-53
  42. Ignacio, R. M., Yoon, Y.S. Sajo, M. E. J., Kim, C. S. Kim, D. H., Kim, S. K., Lee, K. J.(2013) The balneotherapy effect of hydrogen reduced water on UVB-mediated skin injury in hairless mice. Molecular & Cellular Toxicology. 9 (1) 15-21
  43. Ichihara, M. article has not yet been published, but is under the scrutiny of peer review. Reference will be provided as soon as it is published.
 

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